| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MCL-1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the MCL-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MCL-1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q07820 |
| Purity: |
Antigen affinity |
| Description: |
Alternative splicing results in multiple transcript variants, Degradation was blocked in patient-derived tumor cells that lacked FBW7 or had loss-of-function mutations in FBW7, MCL-1is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors, Phosphorylation of MCL-1 directs its interaction with the tumor suppressor protein FBW7, The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products(isoform 2 and isoform 3) promote apoptosis and are death-inducing, The polyubiquitylation of MCL-1 then targets it for proteasomal degradation, Using the methods of somatic cell hybrid analysis and fluorescence in situ hybridization), also known as Myeloid cell leukemia sequence 1, conferring resistance to antitubulin agents and promoting chemotherapeutic-induced polyploidy, encodes an anti-apoptotic protein, the MCL-1 gene is mapped to human 1q21, which is a member of the Bcl-2 family, which is the substrate-binding component of a ubiquitin ligase complex, BCL2L3 |
| Immunogen: |
Amino acids 303-325 (RDWLVKQRGWDGFVEFFHVEDLE-human) were used as the immunogen for this MCL-1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MCL-1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MCL-1 |
| Short name: |
MCL-1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MCL-1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6943 |
| Gene: |
MCL1 |
More about : MCL1 |
| Long gene name: |
BCL2 family apoptosis regulator |
| Synonyms gene name: |
myeloid cell leukemia sequence 1 (BCL2-related) myeloid cell leukemia 1 |
| Synonyms: |
BCL2L3 Mcl-1 |
| Locus: |
1q21, 2 |
| Discovery year: |
1993-11-09 |
| GenBank acession: |
BC017197 |
| Entrez gene record: |
4170 |
| Pubmed identfication: |
7682708 7835896 |
| RefSeq identity: |
NM_021960 |
| Classification: |
BCL2 family |
| Havana BLAST/BLAT: |
OTTHUMG00000034867 |