| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
GAD65 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the GAD65 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This GAD65 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q05329 |
| Purity: |
Antigen affinity |
| Description: |
Bu et al, GAD65 is a target of auto-antibodies in people who later develop type 1 diabetes mellitus or latent auto-immune diabetes, GAD65 is the isoform with molecular weight of 65 kDa, Karlsen et al, The deduced 585-amino acid protein has a molecular mass of 65 kD, (1991) mapped the GAD2 gene to chromosome 10p13-p11, (1992) isolated a cDNA corresponding to the GAD2 gene from a human brain cDNA library, 2 by in situ hybridization, Glutamate decarboxylase or glutamic acid decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2 |
| Immunogen: |
mouse and rat), An amino acid sequence from the N-terminus of the human protein (ENPGTARAWCQVAQKFT) was used as the immunogen for this GAD65 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GAD65 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
GAD65 |
| Short name: |
GAD65 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GAD65 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4093 |
| Gene: |
GAD2 |
More about : GAD2 |
| Long gene name: |
glutamate decarboxylase 2 |
| Synonyms gene name: |
65kD) glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 (pancreatic islets and brain |
| Synonyms: |
GAD65 |
| Locus: |
10p12, 1 |
| Discovery year: |
1991-11-29 |
| GenBank acession: |
AJ251501 |
| Entrez gene record: |
2572 |
| Pubmed identfication: |
2039509 |
| RefSeq identity: |
NM_000818 |
| Havana BLAST/BLAT: |
OTTHUMG00000017836 |