| Long name: |
FITC Conjugated, GAD65 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GAD65 |
| Specificity: |
This is a highly specific antibody against GAD65 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
541-567/585 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human GAD65 |
| Gene ID number: |
2572 |
| Swiss Prot: |
Q05329 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes, Alternative splicing results in multiple transcript variants that encode the same protein, Oct 2008], The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid, This gene may also play a role in the stiff man syndrome, [provided by RefSeq, identified as a major autoantigen in insulin-dependent diabetes, This gene encodes one of several forms of glutamic acid decarboxylase |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
GAD65 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GAD65 Conjugated |
| Short name: |
FITC Conjugated, GAD65 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, GAD65 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4093 |
| Gene: |
GAD2 |
More about : GAD2 |
| Long gene name: |
glutamate decarboxylase 2 |
| Synonyms gene name: |
65kD) glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 (pancreatic islets and brain |
| Locus: |
10p12, 1 |
| Discovery year: |
1991-11-29 |
| GenBank acession: |
AJ251501 |
| Entrez gene record: |
2572 |
| Pubmed identfication: |
2039509 |
| RefSeq identity: |
NM_000818 |
| Havana BLAST/BLAT: |
OTTHUMG00000017836 |