| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
APPL1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the APPL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This APPL1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9UKG1 |
| Purity: |
Antigen affinity |
| Description: |
APPL1 is highly expressed in skeletal muscle, It contains 709 amino acids and shares a 54% amino acid identity with APPL2, It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation, and pancreas, heart, ovary, tissues in which AKT2 mRNA is abundant, DCC-interacting protein 13-alpha is a protein that in humans is encoded by the APPL1 gene |
| Immunogen: |
mouse and rat), An amino acid sequence from the N-terminus of human APPL1 (NDHDAAINRYSRLSKKRENDK) was used as the immunogen for this APPL1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the APPL1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
APPL1 |
| Short name: |
APPL1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
APPL1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
24035 |
| Gene: |
APPL1 |
More about : APPL1 |
| Long gene name: |
adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 |
| Synonyms gene name: |
PH domain and leucine zipper containing 1 , adaptor protein, phosphotyrosine interaction |
| Synonyms: |
APPL |
| Synonyms name: |
DCC-interacting protein 13-alpha |
| Locus: |
3p14, 3 |
| Discovery year: |
2007-01-26 |
| GenBank acession: |
AB037849 |
| Entrez gene record: |
26060 |
| Pubmed identfication: |
10490823 17030088 |
| RefSeq identity: |
NM_012096 |
| Classification: |
Pleckstrin homology domain containing BAR-PH domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000133756 |