| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Podocin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-F, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Podocin antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Podocin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NP85 |
| Purity: |
Antigen affinity |
| Description: |
PDCN is a causative gene for Familial idiopathic nephrotic syndromes, also called NPHS2, and include autosomal recessive steroid-resistant nephrotic syndrome, is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis, which is characterized by early childhood onset of proteinuria, which represents a heterogeneous group of kidney disorders, Podocin (PDCN) |
| Immunogen: |
Amino acids 368-383 (KPVEPLNPKKKDSPML) were used as the immunogen for this Podocin antibody (100% homologous human and mouse) |
| Storage: |
After reconstitution, Avoid repeated freezing and thawing, Celcius or lower, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, The lyophilized Podocin antibody can be stored at 4oC to -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Podocin |
| Short name: |
Podocin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Podocin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
13394 |
| Gene: |
NPHS2 |
More about : NPHS2 |
| Long gene name: |
NPHS2, podocin |
| Synonyms gene name: |
idiopathic, nephrosis 2, steroid-resistant (podocin) |
| Synonyms: |
SRN1 PDCN |
| Locus: |
1q25, 2 |
| Discovery year: |
2000-11-21 |
| GenBank acession: |
AJ279254 |
| Entrez gene record: |
7827 |
| Pubmed identfication: |
8589695 10742096 |
| Havana BLAST/BLAT: |
OTTHUMG00000035252 |
| Locus Specific Databases: |
LRG_887 |