| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NPHS2 (Podocin) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the NPHS2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This NPHS2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NP85 |
| Purity: |
Antigen affinity |
| Description: |
Boute et al, By positional cloning, It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, also called Podocin (PDCN), and encodes a new integral membrane protein, and include autosomal recessive steroid-resistant nephrotic syndrome, belonging to the stomatin protein family, comprising nonsense, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier, frameshift and missense mutations, is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane, it was mapped to 1q25-31, podocin, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis, to segregate with the disease, which is characterized by early childhood onset of proteinuria, which represents a heterogeneous group of kidney disorders, (2000) found ten different NPHS2 mutations, NPHS2 |
| Immunogen: |
An amino acid sequence from the middle region of human NPHS2 (CVKVVQEYERVIIFRLGH) was used as the immunogen for this NPHS2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the NPHS2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NPHS2 (Podocin) |
| Short name: |
NPHS2 Antibody (Podocin) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
NPHS2 (Antibody to) (Podocin) |
| Alternative technique: |
antibodies |
| Identity: |
13394 |
| Gene: |
NPHS2 |
More about : NPHS2 |
| Long gene name: |
NPHS2, podocin |
| Synonyms gene name: |
idiopathic, nephrosis 2, steroid-resistant (podocin) |
| Synonyms: |
SRN1 PDCN |
| Locus: |
1q25, 2 |
| Discovery year: |
2000-11-21 |
| GenBank acession: |
AJ279254 |
| Entrez gene record: |
7827 |
| Pubmed identfication: |
8589695 10742096 |
| Havana BLAST/BLAT: |
OTTHUMG00000035252 |
| Locus Specific Databases: |
LRG_887 |