| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MTCO1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the MTCO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MTCO1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P00395 |
| Purity: |
Antigen affinity |
| Description: |
2 hemes and 2 copper centers, Acin-Perez et al, And they hypothesized that deleterious mutations can arise and become predominant, Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation, II, In mitochondrial Complex IV, It is composed of 13 polypeptides, MTCO2, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, MTCO3) of respiratory Complex IV, Subunits I, The 2 hemes and CuB are bound to subunit I, The cytochrome c oxidase family of enzymes have 4 redox centers, VIIa, VIIb, VIIc, VIa, VIb, VIc, Va, Vb, and III(MTCO1, and VIII are nuclear encoded, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB, the respiratory chain has little spare COX capacity, (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA, Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1 |
| Immunogen: |
An amino acid sequence from the C-terminus of human MTCO1 (PYHTFEEPVYMKS) was used as the immunogen for this MTCO1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MTCO1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MTCO1 |
| Short name: |
MTCO1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MTCO1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7419 |
| Gene: |
MT-CO1 |
More about : MT-CO1 |
| Long gene name: |
mitochondrially encoded cytochrome c oxidase I |
| Synonyms gene: |
MTCO1 |
| Synonyms gene name: |
cytochrome c oxidase I |
| Synonyms: |
COX1 COI |
| Locus: |
mitochondria |
| Discovery year: |
1989-10-12 |
| Entrez gene record: |
4512 |
| Pubmed identfication: |
7219534 |
| RefSeq identity: |
YP_003024028 |
| Classification: |
Mitochondrial complex IV: cytochrome c oxidase subunits |