| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Caspase-3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This Caspase-3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P42574 |
| Purity: |
Antigen affinity |
| Description: |
7, 9, Additionally, And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor, CFLAR, Caspase 8, Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, Deleted in Colorectal Cancer, HCLS1, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis, In erythroid precursors undergoing terminal differentiation, It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, TRAF3 and GroEL, This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family, This protein cleaves and activates caspases 6, XIAP, and 10, and 9, and the protein itself is processed by caspases 8, large and small, that dimerize to form the active enzyme, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease, which is associated with neuronal death in Alzheimers disease, Caspase-3 is a protein which interacts with Survivin |
| Immunogen: |
This amino acid sequence is from the small subunit, An amino acid sequence from the C-terminus of mouse Caspase-3 (CSMLKLYAHKLEFMHIL) was used as the immunogen for this Caspase-3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Caspase-3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Caspase-3 |
| Short name: |
Caspase-3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
caspase-3 (Antibody to) |
| Alternative technique: |
antibodies |