| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Caspase-3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This Caspase-3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P42574 |
| Purity: |
Antigen affinity |
| Description: |
CPP32 or APOPAIN, Coexpression of the two CASP3 subunits in insect cells also resulted in apoptosis, Fernandes-Alnemri et al, It is encoded by the CASP3 gene, Tewari et al, Tiso et al, also known as YAMA, is a member of the cysteine-aspartic acid protease family known to interact with family members -8 and -9, (1994) found that overexpression of CASP3 in insect cells induced apoptosis, (1995) showed that purified human CASP1 cleaved the CASP3 proenzyme into a proteolytically active form and that activated Caspase-3 cleaved PARP into the 85-kD apoptotic form, (1996) used radiation hybrid mapping to localize the gene to human chromosome 4q33-q35, 1, Caspase-3 |
| Immunogen: |
This amino acid sequence is from the small subunit, An amino acid sequence from the C-terminus of the human protein (CAMLKQYADKLEFMHIL) was used as the immunogen for this Caspase-3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Caspase-3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Caspase-3 |
| Short name: |
Caspase-3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
caspase-3 (Antibody to) |
| Alternative technique: |
antibodies |