| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
HIF2a |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the HIF2a antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This HIF2a antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9JHS1 |
| Purity: |
Antigen affinity |
| Description: |
Furthermore, HIF2a binds to and activates transcription from the HIF1a response element derived from the 3-prime flanking region of the EPO gene, HIF2a is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo, In addition, It is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined, Like HIF1a, Moreover, The human gene contains 15 exons and spans at least 120 kb, The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of HIF2a and AHR are similar, The predicted mouse protein is 88% identical to human, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia, it may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event, the predicted protein shares 48% sequence identity with HIF1a, the variation in HIF-2 alpha influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration, HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16 |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human HIF-2 alpha (HALDSENMTKSHQNLCTKGQ) was used as the immunogen for this HIF2a antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HIF2a antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
HIF2a |
| Short name: |
HIF2a Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
HIF2a (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3374 |
| Gene: |
EPAS1 |
More about : EPAS1 |
| Long gene name: |
endothelial PAS domain protein 1 |
| Synonyms: |
MOP2 PASD2 HIF2A HLF bHLHe73 |
| Synonyms name: |
HIF-1 alpha-like factor |
| Locus: |
2p21 |
| Discovery year: |
1998-05-29 |
| GenBank acession: |
U81984 |
| Entrez gene record: |
2034 |
| Pubmed identfication: |
9000051 9079689 18378852 |
| RefSeq identity: |
NM_001430 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000128818 |