| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
GJB2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the GJB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This GJB2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P29033 |
| Purity: |
Antigen affinity |
| Description: |
CX26 shares 92, Connexin 26 gene(GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation, GJB2, Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells, Proteins, The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence, The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD, These structures were shown to consist of cell-to-cell channels, also known as GAP junction protein, beta2, called connexins, connexin 26(GJB2) is assigned to human chromosome 13q11-q12 , purified from fractions of enriched gap junctions from different tissues differ, 5% identity with rat Cx26, Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response, Connexin26(CX26) |
| Immunogen: |
An amino acid sequence from the N-terminus of human GJB2 (MDWGTLQTILGGVNKH) was used as the immunogen for this GJB2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GJB2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
GJB2 |
| Short name: |
GJB2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GJB2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4284 |
| Gene: |
GJB2 |
More about : GJB2 |
| Long gene name: |
gap junction protein beta 2 |
| Synonyms gene: |
DFNB1 DFNA3 |
| Synonyms gene name: |
26kD (connexin 26) gap junction protein, 26kDa , 26kDa (connexin 26) gap junction protein, beta 2, beta 2, beta 2, gap junction protein |
| Synonyms: |
CX26 NSRD1 |
| Synonyms name: |
connexin 26 |
| Locus: |
13q12, 11 |
| Discovery year: |
1990-02-12 |
| GenBank acession: |
M86849 |
| Entrez gene record: |
2706 |
| Pubmed identfication: |
9139825 |
| Classification: |
Gap junction proteins Deafness associated genes |
| Havana BLAST/BLAT: |
OTTHUMG00000016513 |
| Locus Specific Databases: |
Hereditary Hearing Loss Homepage The Connexin-deafness homepage CCHMC-BMI &, UC Hearing Loss Mutation Database |