| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Angiopoietin 2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Angiopoietin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Angiopoietin 2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O15123 |
| Purity: |
Antigen affinity |
| Description: |
ANGPT1 binding to Tie 2 causes phosphorylation of the receptor, Angiopoietin 2 competes for this binding, Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, Angiopoietin 2 is only 60% homologous with -1, It is a naturally occurring antagonist of ANGPT1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation, and thus blocks receptor phosphorylation, ovary, placenta and uterus, Angiopoietin 2 and -1 are involved in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie 2 |
| Immunogen: |
Amino acids 454-468 (NLNGMYYPQRQNTNK-human) were used as the immunogen for this Angiopoietin 2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Angiopoietin 2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Angiopoietin 2 |
| Short name: |
Angiopoietin 2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Angiopoietin 2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
485 |
| Gene: |
ANGPT2 |
More about : ANGPT2 |
| Long gene name: |
angiopoietin 2 |
| Synonyms: |
Ang2 |
| Locus: |
8p23, 1 |
| Discovery year: |
1997-07-22 |
| GenBank acession: |
AF004327 |
| Entrez gene record: |
285 |
| Pubmed identfication: |
9545648 |
| RefSeq identity: |
NM_001147 |
| Classification: |
Fibrinogen C domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000090365 |