| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Occludin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Occludin antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This Occludin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q16625 |
| Purity: |
Antigen affinity |
| Description: |
It has been found that Occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, It is a member of a family of proteins containing the highly conserved Marvel domain, It is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins, Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions, This gene is mapped to 5q13, resulting in RAC1 activation and formation of lamellipodia, which contains 4 transmembrane-helix regions, 2, Occludin is an integral membrane protein that is located at tight junctions |
| Immunogen: |
Human partial recombinant protein (AA 373-522) was used as the immunogen for this Occludin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Occludin antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Occludin |
| Short name: |
Occludin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Occludin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8104 |
| Gene: |
OCLN |
More about : OCLN |
| Long gene name: |
occludin |
| Synonyms: |
PPP1R115 |
| Synonyms name: |
regulatory subunit 115 , tight junction protein occludin TM4 minus phosphatase 1 |
| Locus: |
5q13, 2 |
| Discovery year: |
1998-01-20 |
| GenBank acession: |
U49184 |
| Entrez gene record: |
100506658 |
| Pubmed identfication: |
8601611 |
| RefSeq identity: |
NM_002538 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000099356 |