| Antigen detectable with this ELISA kit: |
Allograft Inflammatory Factor 1 (AIF1) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
1pg/mL, The minimum detectable dose of this kit is typically less than 13 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Allograft Inflammatory Factor 1 (AIF1), The concentration of Allograft Inflammatory Factor 1 (AIF1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Allograft Inflammatory Factor 1 (AIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Allograft Inflammatory Factor 1 (AIF1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Allograft Inflammatory Factor 1 (AIF1) |
| Short name: |
Allograft Inflammatory Factor 1 (AIF1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Allograft Inflammatory Factor 1 (allograft inflammatory factor 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AIF-1 and IBA1 and IRT-1 and IRT1, AIF1 and IDBG-634144 and ENSBTAG00000020554 and 280989, AIF1 and IDBG-78190 and ENSG00000204472 and 199, Aif1 and IDBG-177226 and ENSMUSG00000024397 and 11629, actin filament binding, engulfment and biological process this GO :0006954 and inflammatory response and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0014739 and positive regulation of muscle hyperplasia and biological process this GO :0016601 and Rac protein signal transduction and biological process this GO :0030027 and lamellipodium and cellular component this GO :0030041 and actin filament polymerization and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031668 and cellular response to extracellular stimulus and biological process this GO :0032587 and ruffle membrane and cellular component this GO :0032870 and cellular response to hormone stimulus and biological process this GO :0034097 and response to cytokine and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042995 and cell projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043204 and perikaryon and cellular component this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0048662 and negative regulation of smooth muscle cell proliferation and biological process this GO :0048678 and response to axon injury and biological process this GO :0051015 and actin filament binding and molecular function this GO :0051017 and actin filament bundle assembly and biological process this GO :0051602 and response to electrical stimulus and biological process this GO :0071315 and cellular response to morphine and biological process this GO :0071346 and cellular response to interferon-gamma and biological process this GO :0071447 and cellular response to hydroperoxide and biological process this GO :0071672 and negative regulation of smooth muscle cell chemotaxis and biological process this GO :0071673 and positive regulation of smooth muscle cell chemotaxis and biological process this GO :0090026 and positive regulation of monocyte chemotaxis and biological process this GO :0097178 and ruffle assembly and biological process this GO :1900087 and positive regulation of G1/S transition of mitotic cell cycle and biological process this GO :2000406 and positive regulation of T cell migration and biological process, nuclei, this GO :0001726 and ruffle and cellular component this GO :0001774 and microglial cell activation and biological process this GO :0001891 and pha this GO cytic cup and cellular component this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0003674 and molecular function and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005884 and actin filament and cellular component this GO :0006911 and pha this GO cytosis, this GO :0003674 : molecular_function, this GO :0003674 : molecular_function and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0051015 : actin filament binding, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0051015 : actin filament binding, allograft inflammatory factor 1 |
| Identity: |
352 |
| Gene: |
AIF1 |
More about : AIF1 |
| Long gene name: |
allograft inflammatory factor 1 |
| Synonyms: |
IRT-1 AIF-1 Em:AF129756, 17 IBA1 |
| Synonyms name: |
interferon gamma responsive transcript ionized calcium-binding adapter molecule 1 |
| Locus: |
6p21, 33 |
| Discovery year: |
1997-07-01 |
| GenBank acession: |
U19713 |
| Entrez gene record: |
199 |
| Pubmed identfication: |
8912632 |
| Classification: |
EF-hand domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000031246 |