| Antigen detectable with this ELISA kit: |
Cullin-5 (CUL5) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 563ng/ml-100ng/ml |
| Sensitivity: |
611ng/ml |
| Application: |
Ready to use kit for performing Enzyme-Linked Immunosorbent Assay (ELISA) |
| Sample types tested for this ELISA kit: |
Serum |
| Time for performing t hourse test: |
1-5 hours |
| Test principle: |
", A set of standards is used to build a standard curve from which the amount of the antigen present in the samples and controls can be directly obtained, After a second washing step, The absorbance is measured on a microplate reader, The antigen from the sample and the standards are allowed to bind to the plate, The enzymatic reaction is terminated by addition of the stopping solution, The intensity of the color formed by the enzymatic reaction is directly proportional to the concentration of te antigen in the sample, This assay utilises two monoclonal antibodies of high specificity: A monoclonal antibody against the antigen of interest is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of the antigen is conjugated to horse radish peroxidase (HRP), a, and subsequently incubated with the HRP conjugate, the enzyme substrate is added, washed, The principle of this ELISA kit follows the typical capture in two steps, a, k, sandwich ELISA" |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cullin-5 (CUL5) |
| Short name: |
Cullin-5 (CUL5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cullin-5 (cullin 5) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, CUL5 and IDBG-70098 and ENSG00000166266 and 8065, Cul5 and IDBG-166023 and ENSMUSG00000032030 and 75717, VACM-1 and VACM1, nuclei, protein heterodimerization activity, this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0004872 and receptor activity and molecular function this GO :0005262 and calcium channel activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006970 and response to osmotic stress and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008283 and cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0016032 and viral process and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0031461 and cullin-RING ubiquitin ligase complex and cellular component this GO :0031466 and Cul5-RING ubiquitin ligase complex and cellular component this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0046982 and protein heterodimerization activity and molecular function this GO :0051480 and cytosolic calcium ion homeostasis and biological process this GO :0070588 and calcium ion transmembrane transport and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005262 : calcium channel activity and also this GO :0005515 : protein binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0046982 : protein heterodimerization activity, this GO :0005262 : calcium channel activity, this GO :0005515 : protein binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0046982 : protein heterodimerization activity, 58905 and IDBG-629769 and ENSBTAG00000012882 and 510331, cullin 5 |
| Identity: |
2556 |
| Gene: |
CUL5 |
More about : CUL5 |
| Long gene name: |
cullin 5 |
| Synonyms: |
VACM-1 |
| Locus: |
11q22, 3 |
| Discovery year: |
1998-12-23 |
| GenBank acession: |
X81882 |
| Entrez gene record: |
8065 |
| Pubmed identfication: |
8681378 9037604 |
| Classification: |
Cullins |
| Havana BLAST/BLAT: |
OTTHUMG00000166369 |