| Category: |
Antibody |
| Concentration: |
05% sodium azide, 1 mg/ml BSA (US sourced) and 0, 5 mg/ml in 1X PBS with 0 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Beta Actin Loading Control |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Chicken, Dog , Due to limited knowledge and inability to test the antibody against all known species, Monkey, Mouse (Mus musculus), Rabbit (Oryctolagus cuniculus), Rat, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
Western blot: 1-3ug/ml |
| Notes: |
beta Actin antibody titration may be required for optimal results, Provided assay concentrations are suggestions only |
| Added buffer: |
pH 7, 1X Phosphate Buffered Saline (PBS), 4 |
| Intented use: |
This beta Actin antibody loading controlis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
60 |
| Purity: |
Purified by protein A Affinity Chromatography |
| Description: |
Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins, An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, Beta actin is commonly used as a loading control protein in western blot, There are three main isoforms of actin in vertebrates: alpha, beta and gamma, integrity and motility, typically transfected vs mock or non transfected cell lysate, Actins proteins are highly conserved and involved in cellular structure |
| Immunogen: |
A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody |
| Storage: |
Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Beta Actin Loading |
| Short name: |
Beta Actin Antibody Loading Control |
| Technique: |
antibodies against human proteins, antibodies for, controls, Antibody |
| Alternative name: |
b Actin (Antibody to) Loading reference |
| Alternative technique: |
antibodies |