| Also known as: |
Flag-Tag |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2b |
| Clone: |
G191 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, Mouse (Mus musculus), Rat (Rattus norvegicus), the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, Recognizes over-expressed proteins containing Flag epitope tag (D-tag) fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells |
| Form/Appearance: |
Frozen liquid, 2) with 50% glycerol, Mouse Monoclonal (IgG2b) 1mg/ml in PBS (pH 7 |
| Concentration: |
05mg/0, 05ml |
| Storage and shipping: |
Aliquot to avoid repeated freezing and thawing, Aliquot will be stable at Store the antibody at +4 degrees Celsius, For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap, for 3 months, from date of shipment, Stable for one year the antibody should be stored at -20 degrees Celsius |
| Tested applications:: |
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB) |
| Properties: |
, Also separation of , FLAGS are also used in the isolation of , For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies, It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray, Suitable for in vivo use in cells, This FLAG-tags have the sequence DYKDDDDK motiv, because its mild purification procedure tends not to disrupt such complexes, or , or , overexpressed proteins from cell lysates is done by FLAG go HIS tags, An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, FLAG epitope that is a polypeptide , FLAG octapeptide, These tags are very useful to do protein purification by , affinity chromatography, protein complexes , protein tag , recombinant, recombinant DNA, that can be added to a protein using , with multiple subunits |
| Conjugation: |
Flag |
| French translation: |
anticorps |
| Gene target: |
Tag monoclonal |
| Short name: |
-Tag monoclonal antibody |
| Technique: |
Tags |
| Label: |
Flag |
| Alternative name: |
Flag-detection labelled monoclonal (Antibody to) |
| Alternative technique: |
antibodies |