| We send this reagant on: |
Dry Ice |
| The temperatureerature for storage is: |
Avoid multiple freeze/thaws by aliquoting, The Fluorimetry Probe is light sensitive and should be maintained in amber tubes, C, Store the entire test kit at -80º |
| Technical file: |
more kit-Fluorimetry, pdf |
| SDS or safty data sheet: |
details (MET-5028) GHS, pdf |
| Type of reagent: |
Metabolism Research |
| Related techniques: |
Lipoprotein Metabolism, Phospholipid Assays, Metabolism Research |
| Use of the reagent: |
530-560 nm/em, 585-595 nm, DAG levels in unknown samples are determined based on the provided DAG standard curve, First, Next, The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, The hydrogen peroxide released from this reaction reacts specifically with the test kit&rsquo, kinase is used to phosphorylate DAG in samples, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate, producing hydrogen peroxide, yielding phosphatidic acid, Our DAG (Diacylglycerol) Assay test kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system, s Fluorimetry Probe and is detected at ex |
| Cellular use: |
Suitable for use with cell lysates |
| Protocol information: |
Detection sensitivity of 4uM DAG |
| Reagent properties: |
DAG standard included |
| Additional lab advice: |
NOTE: Each sample replicate requires 2 assays for proper data calculation |
| Conjugation and form: |
host specific |
| Species compatible to: |
human cells and cell lines and other cells |
| Use of this test kit or product: |
Molecular biology |
| Antigen: |
the sequence of the protein |
| Buffer: |
11, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
kiloDalton |
| This reagent is purified: |
98 |
| Test: |
Cellular biology labs supplies other types of Assays as 1 |
| Gene target: |
DAG (Diacylglycerol) test kit |
| Short name: |
DAG (Diacylglycerol) Assay test kit |
| Technique: |
assays, Assay |
| Host: |
Assay |
| Alternative name: |
DAG (Diacylglycerol) test test reagent |
| Alternative technique: |
arrays |
| Identity: |
2666 |
| Gene: |
DAG1 |
More about : DAG1 |
| Long gene name: |
dystroglycan 1 |
| Synonyms gene name: |
dystroglycan 1 (dystrophin-associated glycoprotein 1) |
| Synonyms: |
A3a 156DAG AGRNR DAG |
| Synonyms name: |
alpha-dystroglycan dystrophin-associated glycoprotein-1 beta-dystroglycan |
| Locus: |
3p21, 31 |
| Discovery year: |
1997-07-22 |
| GenBank acession: |
L19711 |
| Entrez gene record: |
1605 |
| Pubmed identfication: |
7774920 1741056 |
| Havana BLAST/BLAT: |
OTTHUMG00000156869 |
| Locus Specific Databases: |
Leiden Muscular Dystrophy pages LRG_854 |