| Also known as: |
H2, KK, MHC Class 1 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a, k |
| Clone: |
36-7-5 |
| Host organism: |
Mouse (Mus musculus) |
| Source organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
Crossreactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis, Does not react with other (e, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, b, d, q) haplotypes, Reacts with the H-2Kk MHC class I alloantigen, g |
| Purification method: |
Purified |
| Form/Appearance: |
0, Conjugated to Biotin, 1% sodium azide, Supplied as a liquid in PBS |
| Storage and shipping: |
Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius, For long-term storage and to avoid repeated freezing and thawing, For maximum recovery of product, Further dilutions can be made in assay buffer, Store the antibody should be stored at -20 degrees Celsius, aliquot and add glycerol (40-50%), centrifuge the original vial after thawing and prior to removing the cap, or colder, Store the antibody at +4 degrees Celsius for short term storage |
| Tested applications:: |
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, Biotin conjugates can be detected by horseradish peroxidase |
| Conjugation: |
Biotinylated |
| Gene target: |
H-2K(K) H2-K1, H2, H2-K), KK (H-2 Class I Histocompatibility K-K alpha Chain, MHC Class 1 |
| Short name: |
H-2K(K) H2-K1, H2, H2-K) ( ), K-K alpha Chain, KK (H-2 Class I Histocompatibility Antigen, MHC Class 1 |
| Label: |
Biotin |
| Alternative name: |
H-2K(K), H2, H2-K) (biotinilated), H2-K1, K-K a epitope, KK (H-2 Class I Histocompatibility protein, Major histocompabitility complex Class 1 |