| known also as: |
X-adsorbed (FITC), Anti-IgG F(ab')2 |
| known also as: |
X-adsorbed (FITC), IgG F(ab')2 |
| known also as: |
X-adsorbed (FITC), IgG F(ab')2 |
| known also as: |
X-adsorbed, IgG F(ab')2 |
| known also as: |
X-adsorbed (FITC), Anti -IgG F(ab')2 |
| Category: |
Antibodies |
| Clonality: |
Polyclonal |
| Immunoglobulin isotype: |
F(ab')2 |
| Clone: |
Polyclonal antibody |
| Host organism: |
Source: Human, Host: Goat |
| Species reactivity: |
Human |
| Specificity and cross-reactivity: |
Human IgG, Human IgG F(ab')2 and Human Serum, No reaction was, anti-Goat Serum, Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein |
| Purification method: |
pepsin digestion and chromatographic separation, Affinity Purified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities |
| Form/Appearance: |
0, Labeled with Fluorescein isothiocyanate (FITC, MW= 390 kD), Reconstitute with 1ml deionized water, and 10mg/ml IgG and protease free bovine serum albumin (BSA), pH 7, 01% sodium azide, 4, Supplied as a powder lyophilized from a solution containing PBS |
| Storage and shipping: |
Aliquots are stable for at least 12 months productone should be stored at -20 degrees Celsius, For long-term storage and to avoid repeated freezing and thawing, For maximum recovery of product, Further dilutions can be made in assay buffer, aliquot and For optimal long term storage, centrifuge the original vial after thawing and prior to removing the cap, productone should be kept at -20 degrees Celsius, Store at 4 degrees Celsius for short term storage |
| Tested for:: |
Immunofluorescence (IF), Western Blot (WB), ELISA (EL/EIA) |
| Description: |
Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions, Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, Properly used, To obtain more detailed information on productone, please, refer to the full product datasheet, the time needed for production is considerably shorter, they are inexpensive to manufacture and respectively to buy, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in, productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, avoid cycles of freezing and thawing, please, In order to retain the quality and the affinity of productone unchanged |
| Group: |
f(ab')2s |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS Polyclonals_1 FITC is highly sensitive to pH extremes, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This MBS Polyclonals_1 Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| About: |
2 light chains, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, The IgG antibody has 2 , There are 2 heavy chains, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, goat polyclonal antibodies , mouse monoclonal H&, Immunoglobulin gamma, L chain clones or rabbit, antibodies, antigen , binding sites, have 4 parts, serum |
| Gene target: |
X-adsorbed |
| Short name: |
X-adsorbed (FITC), IgG F(ab')2 |
| Technique: |
, Fluorescein, IgGs, F(ab')2, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph |
| Isotype: |
IgG, F(ab')2 , Fragment Affinity-Purified Secondary Antibodies |
| Label: |
FITC |
| Alternative name: |
X-adsorbed (fluorecein), Immunoglobulin G F(Antibody')2 |
| Alternative technique: |
secondairy |