| Also known as: |
Biotin |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
Hyb-8 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, All species |
| Specificity and cross-reactivity: |
Anti-biotin antibody may be substituted to decrease this noise, In some applications, It has a very high affinity for biotin and is excellent for use in various amplification techniques, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds, This particular antibody might cross react with speacies outside of the listed ones, localization of biotinylated probes with avidin produces unacceptably high background staining, It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin |
| Storage and shipping: |
Antibody is stable for 24 months, Temperature variations in the range of 1 to 7 degrees Celsius are tolerable, We recommend NOT to freeze the antibody, Store the antibody at +4C |
| Tested applications:: |
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology (IH), In situ Nucleic Acid Hybridization, Western Blot (WB), Confocal Microscopy |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Biotinylated |
| Gene target: |
(Vitamin B7 Vitamin H) |
| Short name: |
(Vitamin B7 Vitamin H) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated (Vitamin B7 and Vitamin H) (Antibody to) |
| Alternative technique: |
antibodies |