| Long name: |
GFP Tag (168AT1211) Monoclonal Antibody |
| Also known as: |
GFP Tag (168AT1211) Monoclonal Antibody |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
GFP Tag |
| Specificity: |
This is a highly specific antibody against GFP Tag |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Monoclonal |
| Clone number: |
168AT1211 |
| Source: |
Purified His-tagged GFP protein was used to produced this monoclonal antibody |
| Tested Applications: |
WB |
| Recommended dilutions: |
WB(1:2000) |
| Cross reactivity: |
Other Species, Human |
| Cross reactive species details: |
All Species |
| Background of the target antigen: |
Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting, Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells, Because of its ability to spontaneously generate its own fluorophore, GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection, However, The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date, This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells, is one of the best visual reporters for monitoring gene expression in vivo and in situ, originally isolated from the jellyfish Aequorea victoria, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology, Green fluorescent protein (GFP) |
| Purification method: |
This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target: |
GFP Tag (168AT1211) Monoclonal |
| Short name: |
GFP Tag (168AT1211) Monoclonal Antibody |
| Technique: |
Monoclonals or monoclonal antibodies, Tags, antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Green Fluorescent detection labelled (168AT1211) monoclonal (Antibody to) |
| Alternative technique: |
antibodies |