| Long name: |
(3A11) Monoclonal Antibody, IKK-&alpha |
| Also known as: |
(3A11) Monoclonal Antibody, IKK-&alpha |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
IKK-&alpha |
| Specificity: |
This is a highly specific antibody against IKK-&alpha |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Monoclonal |
| Clone number: |
3A11 |
| Concentration: |
2ug/ul |
| Source: |
Synthetic peptide |
| Gene ID number: |
1147 |
| Swiss Prot: |
O15111 |
| Tested Applications: |
IP, WB |
| Recommended dilutions: |
0 ug/mL) IP (2 ug), WB (2 |
| Cross reactivity: |
Mouse, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues, Additionally, As part of the non-canonical pathway of NF-kappa-B activation, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation, DNA damages or other cellular stresses, In turn, In turn, Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP, Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK, TAX1BP1, Therefore, These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome, Within the nucleus, and the E3 ligases ITCH and RNF11), bacterial or viral products, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes, or protection against apoptosis, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities, phosphorylates the CREBBP-interacting protein NCOA3, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis, Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines |
| Purification method: |
Purified by Protein G |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
CHUK, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, I-kappa-B kinase alpha, IKBKA, IKK-A, IKK-alpha, IKKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF-16, TCF16, Transcription factor 16, IKK1 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target: |
(3A11) Monoclonal, IKK-&alpha |
| Short name: |
(3A11) Monoclonal Antibody, IKK-&alpha |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
(3A11) monoclonal (Antibody to), IKK-&alpha |
| Alternative technique: |
antibodies |