| Also known as: |
AMPH / Amphiphysin |
| Other names: |
Amphiphysin, N/A, amphiphysin, amphiphysin, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen), amphiphysin I, amphiphysin isoform 1 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
AMPH |
| Other gene names: |
AMPH, AMPH1, AMPH1, AMPH |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG |
| Clone: |
3 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Dog, Due to limited knowledge and inability for testing each and every species, Hamster, Human (Homo sapiens), Monkey, Mouse (Mus musculus), Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus), the reactivity of the antibody may extend to other species which are not listed hereby, Bovine |
| Specificity and cross-reactivity: |
A 108 kD isoform may be observed in some tissues or cell lysates, Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The epitope is located within the carboxyl terminal region of Amphiphysin I (residues 545-695), This particular antibody might cross react with speacies outside of the listed ones, bovine, canine, corresponding to the apparent molecular mass of Amphiphysin I on SDS-PAGE immunoblots, hamster, in samples from human, monkey and rabbit origins, mouse, rat, Detects a ~128 kD protein |
| Purification method: |
Protein G Purified |
| Form/Appearance: |
0, 50% glycerol, Sourced in Ascites, 1 mM PMSF, PBS |
| Concentration: |
1 miligram per 1 mililiter |
| Storage and shipping: |
the antibody should be kept at -20 degrees Celsius, For optimal long term storage |
| Tested applications:: |
Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC - Paraffin) |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About: |
2 light chains, Each murine monoclonal anibody has his own affinity specific for the clone, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, Monoclonals of this antigen are available in different clones, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, The IgG antibody has 2 , There are 2 heavy chains, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, goat polyclonal antibodies , mouse monoclonal H&, Immunoglobulin gamma, L chain clones or rabbit, antibodies, antigen , binding sites, have 4 parts, serum |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
Monoclonal [ 3] AMPH / Amphiphysin |
| Short name: |
Mouse Monoclonal [clone 3] (IgG) AMPH / Amphiphysin Antibody |
| Technique: |
IgGs, Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype: |
IgG |
| Species: |
Humans, Mouses, Human |
| Alternative name: |
sapiens amphiphysin / Amphiphysin (Antibody to), Mouse monoclonal [clonality 3] (Immunoglobulin G) to H |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AMPH and IDBG-12921 and ENSG00000078053 and 273, AMPH and IDBG-634592 and ENSBTAG00000031967 and 614722, AMPH1, Amph and IDBG-132340 and ENSMUSG00000021314 and 218038, Cell surfaces, phospholipid binding, this GO :0005515 and protein binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0006897 and endocytosis and biological process this GO :0007268 and synaptic transmission and biological process this GO :0007612 and learning and biological process this GO :0008021 and synaptic vesicle and cellular component this GO :0015629 and actin cytoskeleton and cellular component this GO :0030054 and cell junction and cellular component this GO :0030672 and synaptic vesicle membrane and cellular component this GO :0031256 and leading edge membrane and cellular component this GO :0048488 and synaptic vesicle endocytosis and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005543 : phospholipid binding, this GO :0005543 : phospholipid binding, amphiphysin |
| Identity: |
471 |
| Gene: |
AMPH |
More about : AMPH |
| Long gene name: |
amphiphysin |
| Synonyms gene name: |
amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) |
| Locus: |
7p14, 1 |
| Discovery year: |
1994-12-12 |
| Entrez gene record: |
273 |
| Pubmed identfication: |
8245793 |
| RefSeq identity: |
NM_001635 |
| Classification: |
N-BAR domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000023725 |