| Also known as: |
Endothelium |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
EN4 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Both large and small vessels are equally reactive, EN4 also binds to guinea-pig and cat endothelium, It stains strongly murine fibroblasts transfected with the human CD31 gene, No other structures in the skin, On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost, This particular antibody might cross react with speacies outside of the listed ones, bovine, but not to rabbit, dog, heart, kidney, rat or mice endothelial cells, sheep, tonsils or spleen are stained with this antibody, This antibody stains all human blood vessels including brain microvessels |
| Purification method: |
Protein G Purified |
| Form/Appearance: |
0, 0, Sourced in TCS, 02% sodium azide, 1% BSA, PBS |
| Storage and shipping: |
Temperature variations in the range between +1C to +7C are tolerable, Keep the antibody refrigerated at +4 degrees Celsius |
| Tested applications:: |
Flow Cytometry (FC/FACS), IHC - Frozen), Immunohistochemistry (IHC - Paraffin |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
Monoclonal [ EN4] (IgG1) Endothelium |
| Short name: |
Mouse Monoclonal [clone EN4] (IgG1) Endothelium Antibody |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype: |
IgG1 |
| Species: |
Humans, Mouses, Human |
| Alternative name: |
sapiens Endothelium (Antibody to), Mouse monoclonal [clonality EN4] (IgG1) to H |
| Alternative technique: |
antibodies |