| Also known as: |
NCAM / CD56 |
| Other names: |
CD_antigen: CD56, NCAM, Neural cell adhesion molecule 1, antigen recognized by monoclonal antibody 5, neural cell adhesion molecule, neural cell adhesion molecule 1, neural cell adhesion molecule 1, 1H11, neural cell adhesion molecule 1 isoform 1 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
NCAM |
| Gene name synonims: |
NCAM1, CD56 |
| Other gene names: |
CD56, MSK39, N-CAM-1, NCAM, NCAM, NCAM-1, NCAM1, NCAM1 |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
123C3 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
123C3 can be used on routing formalin fixed paraffin section after retrieval, 123C3 staining has been associated with more advanced stage and a decreased survival after surgery (Kibbelaar et al, 1991), A minority of cases of other major types of lung carcinoma are sometimes positive as well: however this positivity is generally weak and focal, Adenoid cystic carcinomas of bronchial glands are strongly positive, All cells in small cell carcinomas and carcinoids of the lung are strongly positive for 123C3, At the international Workshop on SCLC antibodies 123C3 has been categorized as cluster 1 antibody (Stahel et al 1994), In non small lung cell carcinomas, It is involved in intercellular adhesion and play a role in outgrowth of neurites and the development of the nervous system, Most importantly, NCAM (CD56) is a marker for natural killer: 123C3 can be used to distinguish NK cells from other hematopoietic cell populations, NKI-nbl-1 (MON 9012) reacts with epitope 1 and 123C3 (MON 9006) reacts with epitope 2, Neuroblastoma's and Wilms tumors are usually also staining strongly positive, Positive staining with other tumors, Several other isoforms have been identified which are expressed in a developmental and tissue specific pattern, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, Two major epitopes have been defined, include medullary thyroid carcinomas and some ovarian tumors, The antibody recognizes a transmembrane glycoprotein of 140 and 180 kD which has been identified as NCAM (Neural Cell Adhesion Module) |
| Purification method: |
Tissue culture supernatant |
| Form/Appearance: |
0, 02% sodium azide, Tissue culture supernatant |
| Storage and shipping: |
For long term storage aliquot and keep the antibody at -20 degrees Celsius, Freeze-thaw cycles should be avoided in order to retain the activity of the antibody, Store at +4 degrees Celsius for short term storage |
| Tested applications:: |
IHC - Frozen), Immunofluorescence (IF), Immunohistochemistry (IHC - Paraffin |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
Monoclonal [ 123C3] (IgG1) NCAM / CD56 |
| Short name: |
Mouse Monoclonal [clone 123C3] (IgG1) NCAM / CD56 Antibody |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype: |
IgG1 |
| Species: |
Humans, Mouses, Human |
| Alternative name: |
sapiens NCAM / CD56 (Antibody to), Mouse monoclonal [clonality 123C3] (IgG1) to H |
| Alternative technique: |
antibodies |
| Identity: |
7656 |
| Gene: |
NCAM1 |
More about : NCAM1 |
| Long gene name: |
neural cell adhesion molecule 1 |
| Synonyms: |
NCAM CD56 |
| Locus: |
11q23, 2 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
4684 |
| RefSeq identity: |
NM_000615 |
| Classification: |
Fibronectin type III domain containing I-set domain containing CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000167196 |