| Also known as: |
CYTOCHROME B245 |
| Other names: |
CGD91-phox, CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b-245 heavy chain, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, beta polypeptide, cytochrome b-245, cytochrome b-245 heavy chain, cytochrome b558 subunit beta, gp91-1, gp91-phox, heme-binding membrane glycoprotein gp91phox, neutrophil cytochrome b 91 kDa polypeptide, p22 phagocyte B-cytochrome, p22 phagocyte B-cytochrome, superoxide-generating NADPH oxidase heavy chain subunit, cytochrome b-245 heavy chain |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
AMCBX2, CGD, CYBB, Cytochrome b558 subunit beta, GP91-1, GP91-PHOX, GP91PHOX, NOX2, NOX2, p91-PHOX, CYBB |
| Gene name synonims: |
AMCBX2, CGD, CYBB, Cytochrome b558 subunit beta, GP91-1, GP91-PHOX, GP91PHOX, NOX2, NOX2, p91-PHOX, CYBB |
| Other gene names: |
AMCBX2, CGD, CYBB, Cytochrome b558 subunit beta, GP91-1, GP91-PHOX, GP91PHOX, NOX2, NOX2, p91-PHOX, CYBB |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
NL7 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Clone NL7 has an inhibitory effect on NADPH oxidase activation, Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons, Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, It recognises the native as well as the denatured form of the protein, NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O<, Research indicates that this inhibitory effect is exerted during the initial stages of oxidase activation, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Superoxide is used for the destruction of pathogens, The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane, This particular antibody might cross react with speacies outside of the listed ones, Upon the appropriate stimulus, _x000D__x000D_Clone NL7 binds amino acids 498-506 (EKDVITGLK), _x000D__x000D_The heavy chain (gp91phox), a cytosolic epitope on gp91phox not accessible on intact neutrophils, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, also known as gp91phox, characterised by severe recurrent infections, or Cyt b), other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex, which is always membrane-resident, <, ) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen, -<, /sub>, /sup>, 2<, CYTOCHROME B245 This item recognises the heavy chain of cytochrome B245, sub>, sup> |
| Form/Appearance: |
Purified (Purified IgG - liquid) |
| Concentration: |
0mg/ml, IgG concentration 1 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody should be stored undiluted, Storage in frost-free freezers is not recommended, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS), Immunohistology - Frozen (IHC), Western Blot (WB), ELISA (EIA) |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Gene target: |
CYTOCHROME B245 HEAVY CHAIN |
| Short name: |
MOUSE Anti-HUMAN CYTOCHROME B245 HEAVY CHAIN Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
sapiens CYTOCHROME B245 HEAVY epitope (Antibody to), MOUSE antibody to-H |
| Alternative technique: |
antibodies |