| Also known as: |
CD8 ALPHA |
| Other names: |
CD8 antigen, Lyt-2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, T-cell surface glycoprotein Lyt-2, alpha chain, 1 lymphocyte differentiation antigen (AA at 100), T-cell surface glycoprotein CD8 alpha chain isoform 1 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD8 ALPHA |
| Other gene names: |
BB154331, Cd8a, Ly-2, Ly-35, Ly-B, Lyt-2, Lyt-2, Lyt2, Cd8a |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
53-6, 7 |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
CD8 is expressed on T-cells, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The CD8 alpha chain specifically binds to class I MHC molecules, This antibody has also been used for depletion of CD8a<, This particular antibody might cross react with speacies outside of the listed ones, _x000D__x000D_CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system, _x000D__x000D_The 53-6, and exists either a homodimer composed of two alpha chains, cells, or a heterodimer composed of one alpha and one beta chain, the alpha chain of the CD8 antigen, +<, /sup>, 7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2, CD8 ALPHA This item detects mouse CD8a, sup> |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
IgG concentration 500ug/1000ul |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Storage in frost-free freezers is not recommended, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Description: |
This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity, Cytotoxic T cells cluster differentiators for flow cytometry |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
CD8 ALPHA: |
| Short name: |
RAT ANTI MOUSE CD8 ALPHA:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
RAT antibody to MOUSE CD8 a:fluorecein (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1706 |
| Gene: |
CD8A |
More about : CD8A |
| Long gene name: |
CD8a molecule |
| Synonyms gene: |
CD8 |
| Synonyms gene name: |
CD8 antigen, alpha polypeptide (p32) T-cell surface glycoprotein CD8 alpha chain |
| Locus: |
2p11, 2 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
925 |
| Pubmed identfication: |
1541829 |
| RefSeq identity: |
NM_001768 |
| Classification: |
CD molecules V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000130265 |
| Locus Specific Databases: |
CD8Abase: Mutation registry for CD8 deficiency LRG_44 |