| Also known as: |
CD107b |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD107b |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
AC17 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Dog |
| Specificity and cross-reactivity: |
CD107b has been shown to share high N-terminal amino acid sequence homology with human, Clone AC17 immunoprecipitates a protein of 95kDa in MDCK cells which, Immunofluorescence staining of MDCK cells with Mouse anti Canine CD107b demonstrates staining patterns consistent with localization to lysozomes, Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The molecular weight of canine CD107b is typical of many lysozome-associated membrane proteins, This is supported by coincident staining of an exogenous lysozomal glycoprotein, This particular antibody might cross react with speacies outside of the listed ones, While most (97%) CD107b resides in the lysozomal environment in adherent MDCK cells <, avian LEP100 transfected into MDCK cells and detected using the anti LEP100 antibody clone CV24, following Endo F digestion to remove N-linked oligosaccharides yields a core protein product of 40kDa, indicating the heavily glycosylated nature of CD107b, is a monoclonal antibody specific for canine CD107b, is found associated with the cell membrane, mouse and rat CD107b, otherwise known as lysosome-associated membrane protein 2 or LAMP-2, the early studies by indicate that a small percentage of total cellular CD107b as revealed by radioimmune assay with clone AC17, they contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion, /em>, /strong>, CD107b <, Mouse anti Dog CD107b (AC17)<, em>, in vitro<, strong> |
| Form/Appearance: |
Purified (Purified IgG - liquid) |
| Concentration: |
0mg/ml, IgG concentration 1 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody should be stored undiluted, Storage in frost-free freezers is not recommended, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB) |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Gene target: |
CD107b |
| Short name: |
MOUSE Anti-DOG CD107b Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MOUSE antibody to-canine CD107b (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6501 |
| Gene: |
LAMP2 |
More about : LAMP2 |
| Long gene name: |
lysosomal associated membrane protein 2 |
| Synonyms gene name: |
lysosomal-associated membrane protein 2 |
| Synonyms: |
CD107b |
| Locus: |
Xq24 |
| Discovery year: |
1990-08-03 |
| GenBank acession: |
X77196 |
| Entrez gene record: |
3920 |
| Classification: |
CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000022301 |
| Locus Specific Databases: |
Mental Retardation database LRG_749 |