| Also known as: |
HLA DR |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
HLA DR |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
YE2/36-HLK |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, DBA, HLA DQ and HLA DR, In humans, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, This item recognises a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, This particular antibody might cross react with speacies outside of the listed ones, and NZB, d and q expressed by mouse strains including C57BL/6, macrophages and activated T lymphocytes, monocytes, this complex is referred to as the human leukocyte antigen (HLA) region, HLA DR The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
HLA DR: |
| Short name: |
RAT ANTI HUMAN HLA DR:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
sapiens Human leukocyte antigen DR:fluorecein (Antibody to), RAT antibody to H |
| Alternative technique: |
antibodies |