| Also known as: |
TCR ALPHA/BETA |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
TCR ALPHA/BETA |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
R73 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Rat (Rattus norvegicus) |
| Specificity and cross-reactivity: |
In the rat thymus, R73 is mitogenic for unseparated spleen cells and for purified T cells, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes, This particular antibody might cross react with speacies outside of the listed ones, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative, TCR ALPHA/BETA This item recognises a constant determinant on the beta chain of the rat alpha/beta T cell receptor |
| Form/Appearance: |
Biotin (Purified IgG conjugated to Biotin - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost-free freezers is not recommended, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Biotinylated |
| Gene target: |
TCR ALPHA/BETA: |
| Short name: |
MOUSE Anti-RAT TCR ALPHA/BETA: Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
Biotin |
| Alternative name: |
MOUSE antibody to-RAT TCR a/b:biotinilated (Antibody to) |
| Alternative technique: |
antibodies |