| Also known as: |
V5-TAG |
| Other names: |
N/A, N/A, Non-structural protein V, V protein, phosphoprotein, V protein |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
V5-TAG |
| Other gene names: |
N/A, N/A, P/V, V/P |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
SV5-Pk1 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Viral |
| Specificity and cross-reactivity: |
(The 9 amino acid epitope is underlined), SV5, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The 14 amino acid epitope is, This item has been used to detect recombinant proteins, This particular antibody might cross react with speacies outside of the listed ones, Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used, gly lys pro <, present on the P/V proteins of the paramyxovirus, ser thr, some of which include transmembrane and secreted proteins, termed Pk, which have been tagged with this epitope, /u>, V5-TAG This item recognises a small epitope, ile pro asn pro leu leu gly leu asp<, u> |
| Form/Appearance: |
Biotin (Purified IgG conjugated to Biotin - liquid) |
| Concentration: |
0 mg/ml, IgG concentration 1 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost-free freezers is not recommended, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
ELISA (EIA), Western Blot (WB), Immunohistology - Frozen (IHC) |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Biotinylated |
| Gene target: |
V5-TAG: |
| Short name: |
MOUSE Anti-V5-TAG: Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
Biotin |
| Alternative name: |
MOUSE antibody to-V5-detection labelled:biotinilated (Antibody to) |
| Alternative technique: |
antibodies |