| Also known as: |
MBP |
| Other names: |
20 kDa microtubule-stabilizing protein, Myelin A1 protein, Myelin basic protein, N/A, N/A, Myelin basic protein |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
MBP |
| Other gene names: |
MBP, MBP, N/A, N/A |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
10 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Bovine |
| Specificity and cross-reactivity: |
<, <, In consequence the antibody is not a suitable reagent for sensitive immunoassays of correct human 82-91 as was thought at the time the original paper on this antibody was published, N, N, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Since this work was done a sequence error has been described in the human MBP sequence in this region Although the clone was selected to have a high affinity for 82-91 it appears that this antibody reacts more strongly with the unnatural sequence, The 91-92 phe-phe bond is believed to be cleaved by demyelinating lesions by Cathepsin D to generate peptides ending in the phe 91 which should react with this monoclonal, The clone was selected using synthetic human MBP peptide 82-91 for screening, This epitope is located in the C terminal region of 82-91 and the corresponding epitope is not present (or exposed) in whole MBP, This particular antibody might cross react with speacies outside of the listed ones, for details, for details, rather than the correct human 82-91, see Groome, see Groome, /i>, /i>, MBP It appears to react with synthetic peptide 82-91 and human and bovine 45-91 but not with whole MBP molecules (Martensen numbering), P, P, et al<, et al<, i>, i> |
| Form/Appearance: |
Ascites (Ascites fluid - liquid) |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
ELISA (EIA), Western Blot (WB), Immunohistology - Frozen (IHC) |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Gene target: |
MBP (aa82-91) |
| Short name: |
MOUSE Anti-MBP (aa82-91) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MOUSE antibody to-MBP (aa82-91) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6925 |
| Gene: |
MBP |
More about : MBP |
| Long gene name: |
myelin basic protein |
| Locus: |
18q23 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
4155 |
| Pubmed identfication: |
2425357 |
| RefSeq identity: |
NM_001025081 |
| Havana BLAST/BLAT: |
OTTHUMG00000132874 |