| Long name: |
FITC Conjugated, MYLK2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MYLK2 |
| Specificity: |
This is a highly specific antibody against MYLK2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human MYLK2 |
| Gene ID number: |
85366 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
CaMKII, CaMKIV and myosin light chain kinases (MYLK, MYLK appears to be a target for PAKs (p21-activated kinases), MYLK2 (also designated skMLCK), Multiple transcript variants are described for the MYLK gene, Non-muscle, PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation, Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues, The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity, The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments, also designated MLCK), smooth muscle and skeletal/cardiac muscle MYLK isoforms exist, The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
skMLCK KMLC MLCK2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MYLK2 Conjugated |
| Short name: |
FITC Conjugated, MYLK2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, myosin light epitope kinase 2 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
MYLK2 and IDBG-64011 and ENSG00000101306 and 85366, MYLK2 and IDBG-641556 and ENSBTAG00000014930 and 533378, Mylk2 and IDBG-209914 and ENSMUSG00000027470 and 228785, nuclei, this GO :0004672 and protein kinase activity and molecular function this GO :0004683 and calmodulin-dependent protein kinase activity and molecular function this GO :0004687 and myosin light chain kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006941 and striated muscle contraction and biological process this GO :0007274 and neuromuscular synaptic transmission and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0014816 and satellite cell differentiation and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004683 : calmodulin-dependent protein kinase activity and also this GO :0004687 : myosin light chain kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005516 : calmodulin binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004683 : calmodulin-dependent protein kinase activity, this GO :0004687 : myosin light chain kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005516 : calmodulin binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018107 and peptidyl-threonine phosphorylation and biological process this GO :0030017 and sarcomere and cellular component this GO :0032971 and regulation of muscle filament sliding and biological process this GO :0035914 and skeletal muscle cell differentiation and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0055008 and cardiac muscle tissue morphogenesis and biological process this GO :0060048 and cardiac muscle contraction and biological process, myosin light chain kinase 2 |
| Identity: |
16243 |
| Gene: |
MYLK2 |
More about : MYLK2 |
| Long gene name: |
myosin light chain kinase 2 |
| Synonyms gene name: |
myosin light chain kinase 2, skeletal muscle |
| Synonyms name: |
skeletal muscle myosin light chain kinase |
| Locus: |
20q11, 21 |
| Discovery year: |
2001-07-31 |
| GenBank acession: |
AF325549 |
| Entrez gene record: |
85366 |
| RefSeq identity: |
NM_033118 |
| Havana BLAST/BLAT: |
OTTHUMG00000032194 |
| Locus Specific Databases: |
LRG_392 |