| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MYLK2 |
| Specificity: |
This antibody reacts specifically with MYLK2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MYLK2 |
| Gene ID: |
85366 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CaMKII, CaMKIV and myosin light chain kinases (MYLK, MYLK appears to be a target for PAKs (p21-activated kinases), MYLK2 (also designated skMLCK), Multiple transcript variants are described for the MYLK gene, Non-muscle, PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation, Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues, The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity, The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments, also designated MLCK), smooth muscle and skeletal/cardiac muscle MYLK isoforms exist, The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
skMLCK KMLC MLCK2 |
| Also known as: |
MYLK2 Polyclonal Antibody |
| Other name: |
Anti-MYLK2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MYLK2 |
| Short name: |
Anti-MYLK2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-myosin light epitope kinase 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
MYLK2 and IDBG-64011 and ENSG00000101306 and 85366, MYLK2 and IDBG-641556 and ENSBTAG00000014930 and 533378, Mylk2 and IDBG-209914 and ENSMUSG00000027470 and 228785, nuclei, this GO :0004672 and protein kinase activity and molecular function this GO :0004683 and calmodulin-dependent protein kinase activity and molecular function this GO :0004687 and myosin light chain kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006941 and striated muscle contraction and biological process this GO :0007274 and neuromuscular synaptic transmission and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0014816 and satellite cell differentiation and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004683 : calmodulin-dependent protein kinase activity and also this GO :0004687 : myosin light chain kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005516 : calmodulin binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004683 : calmodulin-dependent protein kinase activity, this GO :0004687 : myosin light chain kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005516 : calmodulin binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018107 and peptidyl-threonine phosphorylation and biological process this GO :0030017 and sarcomere and cellular component this GO :0032971 and regulation of muscle filament sliding and biological process this GO :0035914 and skeletal muscle cell differentiation and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0055008 and cardiac muscle tissue morphogenesis and biological process this GO :0060048 and cardiac muscle contraction and biological process, myosin light chain kinase 2 |
| Identity: |
16243 |
| Gene: |
MYLK2 |
More about : MYLK2 |
| Long gene name: |
myosin light chain kinase 2 |
| Synonyms gene name: |
myosin light chain kinase 2, skeletal muscle |
| Synonyms name: |
skeletal muscle myosin light chain kinase |
| Locus: |
20q11, 21 |
| Discovery year: |
2001-07-31 |
| GenBank acession: |
AF325549 |
| Entrez gene record: |
85366 |
| RefSeq identity: |
NM_033118 |
| Havana BLAST/BLAT: |
OTTHUMG00000032194 |
| Locus Specific Databases: |
LRG_392 |