| Long name: |
FITC Conjugated, Nidogen 2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Nidogen 2 |
| Specificity: |
This is a highly specific antibody against Nidogen 2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Secreted, Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Nidogen 2 |
| Gene ID number: |
22795 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
IV and Perlecan, In mammals, Nidogen and Nidogen-2, Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane, The two isoforms have a similar shape, and co-localize in vessel walls and other basement membrane zones, and is widely distributed in basement membranes in heart, bone and, consisting of three globular domains, in pancreas, interact at comparable levels with Collagen I, kidney and skeletal muscle, placenta, serving to stabilize basement membranes and playing a major role in embryogenesis, to a lesser extent, two Nidogen proteins, Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
NID 2, NID2, Nidogen2, Osteonidogen |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Nidogen 2 Conjugated |
| Short name: |
FITC Conjugated, Nidogen 2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Nidogen 2 (Antibody to) |
| Alternative technique: |
antibodies |