| Long name: |
FITC Conjugated, HLA B27 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HLA B27 |
| Specificity: |
This is a highly specific antibody against HLA B27 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human HLA B27 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum, HLA-B and HLA-C) and class II (HLA-D), HLA-B encodes a membrane anchored heavy chain which hetero-dimerizes with a light chain (â, Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor, Polymorphisms yield hundreds of HLA-B alleles, The HLA-B27 allele appears with increased frequency in uveitis patients, The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes, There are two classes of HLA antigens: class I (HLA-A, They are cell-surface receptors that bind peptides and present them to T lymphocytes, &circ, «, -2-Microglobulin) to form MHC-I, Major histocompatibility complex (MHC) molecules form an integral part of the immune response system |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
1B27_HUMAN, HLAB, Leukocyte antigen class I B, Lymphocyte antigen, Major histocompatibility complex class I B, SPDA1, HLA B27 HLA-B27 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HLA B27 Conjugated |
| Short name: |
FITC Conjugated, HLA B27 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Human leukocyte antigen B27 (Antibody to) |
| Alternative technique: |
antibodies |