| Long name: |
ALEXA FLUOR 350 Conjugated, GCLC Polyclonal Antibody |
| Also known as: |
Anti-GCLC PAb ALEXA FLUOR 350 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
350, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GCLC |
| Specificity: |
This is a highly specific antibody against GCLC |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human GCLC |
| Gene ID Number: |
2730 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels, Cadium regulates transcription through proteins Met-4, Cbf1, Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, H2O2 regulation depends on the Yap1 protein and the presence of glutamate, Met-31 and Met-32, Oxidants, a DNA binding protein, also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC), brain, cadium and methyl mercury upregulate the transcription of g-GCS, g-GCS, g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis, g-GCSc associates with a regulatory or modifier subunit, g-GCSc is expressed in hemocytes, g-GCSm (g-glutamylcysteine synthetase light subunit), glutamine and lysine, inhibits transcription of g-GCS, liver and kidney, to form a heterodimer, which may protect against chemically induced carcinogenesis, Defects in GCLC are the cause of hemolytic anemia, The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit) |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
343nm/442nm |
| Synonyms: |
GCS |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 350, Alexa Fluor |
| Gene target: |
GCLC |
| Short name: |
GCLC Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 350 |
| Alternative name: |
ALEXA FLUOR 350, catalytic subunit (Antibody to), glutamate-cysteine ligase |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Cytoplasm, DNA-templated and biological process this GO :0046685 and response to arsenic-containing substance and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0050662 and coenzyme binding and molecular function this GO :0050880 and regulation of blood vessel size and biological process this GO :0051409 and response to nitrosative stress and biological process this GO :0051900 and regulation of mitochondrial depolarization and biological process this GO :1901687 and glutathione derivative biosynthetic process and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, GCL and GCS and GLCL and GLCLC, GCLC and IDBG-628748 and ENSBTAG00000015571 and 512468, GCLC and IDBG-91041 and ENSG00000001084 and 102724876, Gclc and IDBG-186028 and ENSMUSG00000032350 and 14629, catalytic subunit, coenzyme binding, this GO :0000096 and sulfur amino acid metabolic process and biological process this GO :0000287 and magnesium ion binding and molecular function this GO :0004357 and glutamate-cysteine ligase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006534 and cysteine metabolic process and biological process this GO :0006536 and glutamate metabolic process and biological process this GO :0006749 and glutathione metabolic process and biological process this GO :0006750 and glutathione biosynthetic process and biological process this GO :0006805 and xenobiotic metabolic process and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0009408 and response to heat and biological process this GO :0009410 and response to xenobiotic stimulus and biological process this GO :0009725 and response to hormone and biological process this GO :0016595 and glutamate binding and molecular function this GO :0017109 and glutamate-cysteine ligase complex and cellular component this GO :0019852 and L-ascorbic acid metabolic process and biological process this GO :0031397 and negative regulation of protein ubiquitination and biological process this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0034641 and cellular nitrogen compound metabolic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0043531 and ADP binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0045892 and negative regulation of transcription, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004357 : glutamate-cysteine ligase activity and also this GO :0005524 : ATP binding and also this GO :0016595 : glutamate binding and also this GO :0043531 : ADP binding and also this GO :0046982 : protein heterodimerization activity and also this GO :0050662 : coenzyme binding, this GO :0004357 : glutamate-cysteine ligase activity, this GO :0005524 : ATP binding, this GO :0016595 : glutamate binding, this GO :0043531 : ADP binding, this GO :0046982 : protein heterodimerization activity, this GO :0050662 : coenzyme binding, 2729, glutamate-cysteine ligase |
| Identity: |
4311 |
| Gene: |
GCLC |
More about : GCLC |
| Long gene name: |
glutamate-cysteine ligase catalytic subunit |
| Synonyms gene: |
GLCLC GLCL |
| Synonyms gene name: |
catalytic subunit , glutamate-cysteine ligase |
| Locus: |
6p12, 1 |
| Discovery year: |
1993-11-24 |
| GenBank acession: |
M90656 |
| Entrez gene record: |
2729 |
| Pubmed identfication: |
1350904 |
| Havana BLAST/BLAT: |
OTTHUMG00000160220 |