| Long name: |
FITC Conjugated, NEDD4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
NEDD4 |
| Specificity: |
This is a highly specific antibody against NEDD4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
861-890/1319 |
| Source: |
KLH conjugated synthetic peptide derived from human NEDD4 |
| Gene ID number: |
4734 |
| Swiss Prot: |
P46934 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable, Involved in the budding of many viruses, Involved in the pathway leading to the degradation of VEGFR-2/KDFR, Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding, Involved in ubiquitination of ERBB4 intracellular domain E4ICD, Monoubiquitinates IGF1R at multiple sites, Part of a signaling complex composed of NEDD4, Promotes ubiquitination of RAPGEF2, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development, Ubiquitinates FGFR1, Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2, independently of its ubiquitin-ligase activity, leading to receptor internalization and degradation in lysosomes, thus leading to receptor internalization and degradation in lysosomes, E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
KIAA0093 MGC176705 NEDD4-1 RPF1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
NEDD4 Conjugated |
| Short name: |
FITC Conjugated, NEDD4 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, NEDD4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7727 |
| Gene: |
NEDD4 |
More about : NEDD4 |
| Long gene name: |
E3 ubiquitin protein ligase , developmentally down-regulated 4, neural precursor cell expressed |
| Synonyms gene name: |
developmentally down-regulated 4 , neural precursor cell expressed |
| Synonyms name: |
receptor-potentiating factor 1 |
| Locus: |
15q21, 3 |
| Discovery year: |
1994-01-21 |
| GenBank acession: |
D42055 |
| Entrez gene record: |
4734 |
| Pubmed identfication: |
9073511 8649367 |
| RefSeq identity: |
NM_198400 |
| Havana BLAST/BLAT: |
OTTHUMG00000132015 |