| Long name: |
FITC Conjugated, DFFB Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
DFFB |
| Specificity: |
This is a highly specific antibody against DFFB |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human DFFB |
| Gene ID number: |
1677 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined, DFF becomes activated when DFFA is cleaved by caspase-3, DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis, DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis, DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits, Jul 2008], The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units, The cleaved fragments of DFFA dissociate from DFFB, [provided by RefSeq, the active component of DFF, Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CAD CPAN DFF-40 DFF40 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
DFFB Conjugated |
| Short name: |
FITC Conjugated, DFFB Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
40kDa, beta polypeptide (caspase-activated DNase) (Antibody to), fluorecein coupled, Desoxyribonucleic acid fragmentation factor |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
40kDa, BT, CAD and CPAN and DFF-40 and DFF2 and DFF40, DFFB and IDBG-86952 and ENSG00000169598 and 1677, Dffb and IDBG-206807 and ENSMUSG00000029027 and 13368, beta polypeptide (caspase-activated DNase), enzyme binding, nuclei, this GO :0000790 and nuclear chromatin and cellular component this GO :0003677 and DNA binding and molecular function this GO :0004518 and nuclease activity and molecular function this GO :0004536 and deoxyribonuclease activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006309 and apoptotic DNA fragmentation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006921 and cellular component disassembly involved in execution phase of apoptosis and biological process this GO :0007420 and brain development and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0030263 and apoptotic chromosome condensation and biological process, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0004518 : nuclease activity and also this GO :0004536 : deoxyribonuclease activity and also this GO :0005515 : protein binding and also this GO :0016787 : hydrolase activity and also this GO :0019899 : enzyme binding, this GO :0004518 : nuclease activity, this GO :0004536 : deoxyribonuclease activity, this GO :0005515 : protein binding, this GO :0016787 : hydrolase activity, this GO :0019899 : enzyme binding, 22809 and IDBG-634397 and ENSBTAG00000020015 and 512730, DNA fragmentation factor |
| Identity: |
2773 |
| Gene: |
DFFB |
More about : DFFB |
| Long gene name: |
DNA fragmentation factor subunit beta |
| Synonyms gene name: |
40kDa, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) |
| Synonyms name: |
caspase-activated DNase |
| Locus: |
1p36, 32 |
| Discovery year: |
1997-10-27 |
| Entrez gene record: |
1677 |
| Pubmed identfication: |
9108473 9560346 |
| RefSeq identity: |
NM_001282669 |
| Havana BLAST/BLAT: |
OTTHUMG00000003525 |