| Long name: |
ALEXA FLUOR 488 Conjugated, SIRT4 Polyclonal Antibody |
| Also known as: |
Anti-SIRT4 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
SIRT4 |
| Specificity: |
This is a highly specific antibody against SIRT4 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
60-110/314 |
| Subcellular location: |
Mitochondrion |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SIRT4/SIR 2 like protein 4 |
| Gene ID Number: |
23409 |
| Swiss Prot: |
Q9Y6E7 |
| Tested applications: |
IF(IHC-F), IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-F)(1:50-200), IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, Acts as a tumor suppressor, Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, Catalyzes the transfer of ADP-ribosyl groups onto target proteins, Down-regulates insulin secretion, In response to mTORC1 signal, SIRT4 expression is repressed, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity, inhibiting its activity, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest, promoting anaplerosis and cell proliferation, thereby acting as a regulator of lipid homeostasis, Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
SIR2L4 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the SIRT4 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
SIRT4 |
| Short name: |
SIRT4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, SIRT4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
14932 |
| Gene: |
SIRT4 |
More about : SIRT4 |
| Long gene name: |
sirtuin 4 |
| Synonyms gene name: |
S, cerevisiae, cerevisiae) , homolog) 4 sirtuin (silent mating type information regulation 2 homolog) 4 (S, sirtuin (silent mating type information regulation 2 |
| Locus: |
12q24, 31 |
| Discovery year: |
2001-03-20 |
| GenBank acession: |
AF083109 |
| Entrez gene record: |
23409 |
| Pubmed identfication: |
10381378 |
| RefSeq identity: |
NM_012240 |
| Classification: |
Sirtuins |
| Havana BLAST/BLAT: |
OTTHUMG00000169028 |