| Long name: |
Biotin Conjugated, CD45 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CD45 |
| Specificity: |
This is a highly specific antibody against CD45 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
475-525/806 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from Bovine CD45/B220 |
| Gene ID number: |
5788 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Bovine |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
180 kDa) which share a common large intracellular domain, 190, 205, CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells, It is likely that CD45 plays an important role in signal transduction, It is not detected on differentiated cells of other tissues, The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1, Their extracellular domains are heavily glycosylated, inhibition or upregulation of various immunological functions, recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc, CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
LCA T200 GP180 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CD45 Conjugated |
| Short name: |
Conjugated, CD45 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, CD45 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9666 |
| Gene: |
PTPRC |
More about : PTPRC |
| Long gene name: |
protein tyrosine phosphatase, receptor type C |
| Synonyms gene: |
CD45 |
| Locus: |
1q31, 1 , 3-q32 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
Y00062 |
| Entrez gene record: |
5788 |
| Pubmed identfication: |
2169617 |
| RefSeq identity: |
NM_002838 |
| Classification: |
Fibronectin type III domain containing CD molecules Protein tyrosine phosphatases, receptor type |
| Havana BLAST/BLAT: |
OTTHUMG00000035702 |
| Locus Specific Databases: |
PTPRCbase: Mutation registry for CD45 deficiency LRG_95 |