| Long name: |
FITC Conjugated, eIF4EBP1+2+3(Thr45) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
e4EBP1+2+3 Thr45 |
| Specificity: |
This is a highly specific antibody against e4EBP1+2+3 Thr45 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr45 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of Thr45 |
| Gene ID number: |
1978 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Interaction of this protein with eIF4E inhibits complex assembly and represses translation, Jul 2008], The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, [provided by RefSeq, resulting in its dissociation from eIF4E and activation of mRNA translation, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs, This gene encodes one member of a family of translation repressor proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
4E BP1, 4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, BP1, Eukaryotic translation initiation factor 4E binding protein 1, Eukaryotic translation initiation factor 4E binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, MGC4316, PHAS, PHAS I, PHAS-I, PHASI, Phosphorylated heat and acid stable protein regulated by insulin 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, e4E binding protein 1, e4E-binding protein 1, e4EBP1, e4EBP1 phospho T36, e4EBP1 phospho Thr36, p-4EBP1/2/3Thr 45, p-e4EBP1 Thr36, p-4E-BP1/2/3 Thr 45 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
eIF4EBP1+2+3(Thr45) Conjugated |
| Short name: |
FITC Conjugated, eIF4EBP1+2+3(Thr45) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, eIF4EBP1+2+3(Thr45) (Antibody to) |
| Alternative technique: |
antibodies |