| Long name: |
FITC Conjugated, p70 S6 kinase alpha Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
p70 S6 kinase alpha |
| Specificity: |
This antibody may detect p70 ribosomal S6 kinase beta 2, This is a highly specific antibody against p70 S6 kinase alpha |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
280-330/525 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human p70 S6 kinase alpha |
| Gene ID number: |
6198 |
| Swiss Prot: |
P23443 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also controls translation initiation by phosphorylating a negative regulator of EIF4A, Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, In cells lacking functional TSC1-2 complex, In response to IGF1, Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function, PDCD4, Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex, Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR, RPS6 and EEF2K, Regulates protein synthesis through phosphorylation of EIF4B, The active form then phosphorylates and activates several substrates in the pre-initiation complex, The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, Under conditions of nutrient depletion, Upon mitogenic stimulation, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), and contributes to cell survival by repressing the pro-apoptotic function of BAD, cell growth and cell cycle progression, constitutively phosphorylates and inhibits GSK3B, including the EIF2B complex and the cap-binding complex component EIF4B, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation, resulting in accelerated degradation of IRS1, resulting in the inhibition of mTORC2 and AKT1 signaling, targeting it for ubiquitination and subsequent proteolysis, the inactive form associates with the EIF3 translation initiation complex, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function, which leads to its inhibition and thus activation of EEF2, Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
70 kDa ribosomal protein S6 kinase 1, P70S6K1, PS6K, RPS6KB1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, STK14A, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha, p70-S6K, p70-S6K 1, p70-alpha, S6K |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
p70 S6 kinase alpha Conjugated |
| Short name: |
FITC Conjugated, p70 S6 kinase alpha Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, p70 S6 phosphorylation catalyst a (Antibody to) |
| Alternative technique: |
antibodies |