| Long name: |
Cy7 Conjugated, p70 S6 kinase alpha Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Cy7 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
p70 S6 kinase alpha |
| Specificity: |
This antibody may detect p70 ribosomal S6 kinase beta 2, This is a highly specific antibody against p70 S6 kinase alpha |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
280-330/525 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human p70 S6 kinase alpha |
| Gene ID number: |
6198 |
| Swiss Prot: |
P23443 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also controls translation initiation by phosphorylating a negative regulator of EIF4A, Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, In cells lacking functional TSC1-2 complex, In response to IGF1, Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function, PDCD4, Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex, Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR, RPS6 and EEF2K, Regulates protein synthesis through phosphorylation of EIF4B, The active form then phosphorylates and activates several substrates in the pre-initiation complex, The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, Under conditions of nutrient depletion, Upon mitogenic stimulation, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), and contributes to cell survival by repressing the pro-apoptotic function of BAD, cell growth and cell cycle progression, constitutively phosphorylates and inhibits GSK3B, including the EIF2B complex and the cap-binding complex component EIF4B, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation, resulting in accelerated degradation of IRS1, resulting in the inhibition of mTORC2 and AKT1 signaling, targeting it for ubiquitination and subsequent proteolysis, the inactive form associates with the EIF3 translation initiation complex, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function, which leads to its inhibition and thus activation of EEF2, Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
743nm/767nm |
| Synonyms: |
70 kDa ribosomal protein S6 kinase 1, P70S6K1, PS6K, RPS6KB1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, STK14A, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha, p70-S6K, p70-S6K 1, p70-alpha, S6K |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Conjugated: |
These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths |
| Gene target: |
p70 S6 kinase alpha Conjugated |
| Short name: |
Conjugated, p70 S6 kinase alpha Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy7 |
| Alternative name: |
cyanine 7 coupled, p70 S6 phosphorylation catalyst a (Antibody to) |
| Alternative technique: |
antibodies |