| Long name: |
Smad3 (Ser208) Primary Polyclonal Antibody |
| Also known as: |
Smad3 (Ser208) PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
Smad3 Ser208 |
| Specificity: |
This is a highly specific antibody against Smad3 Ser208 |
| Modification(s): |
Phosphorylation |
| Modification site(s): |
Ser208 |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser208 [NL(p-S)PN] |
| Gene ID number: |
4088 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
(Smad4) that functions via its interaction to the various Smads, (Smad6 and 7), 5, Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling, The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, allowing its binding to DNA and transcription factors, and 8), and the inhibitory Smads, differentiation and development, the common partner, Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
HSPC193, JV15 2, JV152, MAD mothers against decapentaplegic Drosophila homolog 3, MAD3, MADH 3, MADH3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD-3, SMAD3_HUMAN, SMAD3phospho Ser208, hMAD 3, hSMAD3, p-SMAD3Ser208, SMAD3phospho S208 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Smad3 (Ser208) |
| Short name: |
Smad3 (Ser208) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SMAD family member 3 (Ser208) (Antibody to) |
| Alternative technique: |
antibodies |