| Long name: |
Cy3 Conjugated, Insulin Receptor Beta (Tyr1185) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Insulin Receptor Beta Tyr1185 |
| Specificity: |
This is a highly specific antibody against Insulin Receptor Beta Tyr1185 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr1185 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1160-1200/1382 |
| Subcellular location: |
Cell membrane |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human INSR around the phosphorylation site of Tyr1185 [DI(p-Y)ET] |
| Gene ID number: |
3643 |
| Swiss Prot: |
P06213 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
IRS1, IRS2 and Shc, RTKs are essential components of signal transduction pathways that affect cell proliferation, RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, Receptor activation occurs through ligand binding, The beta subunit (95 kDa) possesses a single transmembrane domain, The insulin receptor exhibits receptor tyrosine kinase (RTK) activity, The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, These three phosphorylated proteins then interact with SH2 domain containing signaling proteins, Three adapter proteins, become phosphorylated on tyrosine residues following insulin receptor activation, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates, differentiation, fibroblast growth factor and vascular endothelial growth factor, migration and metabolism, whereas the alpha subunit (135 kDa) is completely extracellular, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion, which then undergoes an autophosphorylation that increases its tyrosine kinase activity, Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
CD22, INSR, IR, Insulin receptor, HHF5 |
| Properties: |
1 of the Insulin Receptor Beta (Tyr1185) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Insulin Receptor Beta (Tyr1185) Conjugated |
| Short name: |
Conjugated, Insulin Receptor Beta (Tyr1185) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, Insulin Receptor b (Tyr1185) (Antibody to) |
| Alternative technique: |
antibodies |