| Long name: |
Biotin Conjugated, ASK1 (Ser83) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ASK1 Ser83 |
| Specificity: |
This is a highly specific antibody against ASK1 Ser83 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser83 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Ser83 |
| Gene ID number: |
4217 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Drosophila, MAPK kinase (MKK or MEK), MAPK kinase/MEK, MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPKK4) in vitro, MAPKKK5 contains 1, MAPKKK5 does not activate MAPK/ERK, Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas, The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, The kinases of these signaling cascades are highly conserved, and MAPK kinase kinase (MAPKKK or MEKK), and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, and homologs exist in yeast, and mammalian cells, which in turn activates MAPK, 374 amino acids with all 11 kinase subdomains, Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
MAPKKK5 ASK1 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ASK1 (Ser83) Conjugated |
| Short name: |
Conjugated, ASK1 (Ser83) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, ASK1 (Ser83) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6857 |
| Gene: |
MAP3K5 |
More about : MAP3K5 |
| Long gene name: |
mitogen-activated protein kinase kinase kinase 5 |
| Synonyms gene: |
MEKK5 |
| Synonyms name: |
apoptosis signal regulating kinase 1 |
| Locus: |
6q23, 3 |
| Discovery year: |
1997-11-14 |
| GenBank acession: |
U67156 |
| Entrez gene record: |
4217 |
| Pubmed identfication: |
9465908 |
| Classification: |
Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000015647 |
| Locus Specific Databases: |
The Globin Gene Server |