| Long name: |
Biotin Conjugated, GSK-3 Beta(Ser21) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GSK-3 Beta Ser21 |
| Specificity: |
This is a highly specific antibody against GSK-3 Beta Ser21 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser21 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
13-23/420 |
| Subcellular location: |
Cell membrane, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21 |
| Gene ID number: |
2932 |
| Swiss Prot: |
P49841 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
APC, AXIN1, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes, CTNNB1/beta-catenin, DPYSL2/CRMP2, EIF2B, GSK3B forms a multimeric complex with APC, In Wnt signaling, In skeletal muscle, JUN, MAPT/TAU and MACF1, MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease, May also mediate the development of insulin resistance by regulating activation of transcription factors, NFATC1/NFATC, Phosphorylates JUN at sites proximal to its DNA-binding domain, Phosphorylates MACF1, Phosphorylates MAPT/TAU on 'Thr-548', Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex, Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA), Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase, Requires primed phosphorylation of the majority of its substrates, Wnt signaling and regulation of transcription factors and microtubules, and thereby opposing the action of calcineurin, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis, decreasing significantly MAPT/TAU ability to bind and stabilize microtubules, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair, shutting off NFATC1/NFATC gene regulation, thereby reducing its affinity for DNA, Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
GSK-3 beta, GSK3B, Serine/threonine-protein kinase GSK3B, Glycogen synthase kinase-3 beta |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GSK-3 Beta(Ser21) Conjugated |
| Short name: |
Conjugated, GSK-3 Beta(Ser21) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, GSK-3 b(Ser21) (Antibody to) |
| Alternative technique: |
antibodies |