| Long name: |
FITC Conjugated, ECT2 (Thr373) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ECT2 Thr373 |
| Specificity: |
This is a highly specific antibody against ECT2 Thr373 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr373 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human ECT2 around the phosphorylation site of Thr373 |
| Gene ID number: |
1894 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver, The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases, Thus, [provided by RefSeq], and is thought to have an important role in the regulation of cytokinesis, this protein is expressed in a cell cycle-dependent manner during liver regeneration, The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ARHGEF31 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ECT2 (Thr373) Conjugated |
| Short name: |
FITC Conjugated, ECT2 (Thr373) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, epithelial cell transforming sequence 2 oncogene (Thr373) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ARHGEF31, BT, ECT2 and IDBG-65239 and ENSG00000114346 and 1894, Ect2 and IDBG-134125 and ENSMUSG00000027699 and 13605, nuclei, protein homodimerization activity, this GO :0000902 and cell morphogenesis and biological process this GO :0000910 and cytokinesis and biological process this GO :0004871 and signal transducer activity and molecular function this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005089 and Rho guanyl-nucleotide exchange factor activity and molecular function this GO :0005096 and GTPase activator activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0005923 and tight junction and cellular component this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0015031 and protein transport and biological process this GO :0017048 and Rho GTPase binding and molecular function this GO :0030496 and midbody and cellular component this GO :0032147 and activation of protein kinase activity and biological process this GO :0032154 and cleavage furrow and cellular component this GO :0032321 and positive regulation of Rho GTPase activity and biological process this GO :0032467 and positive regulation of cytokinesis and biological process this GO :0032862 and activation of Rho GTPase activity and biological process this GO :0032863 and activation of Rac GTPase activity and biological process this GO :0035023 and regulation of Rho protein signal transduction and biological process this GO :0042307 and positive regulation of protein import into nucleus and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043089 and positive regulation of Cdc42 GTPase activity and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0045666 and positive regulation of neuron differentiation and biological process this GO :0045859 and regulation of protein kinase activity and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051056 and regulation of small GTPase mediated signal transduction and biological process this GO :0051260 and protein homooli this GO merization and biological process this GO :0051988 and regulation of attachment of spindle microtubules to kinetochore and biological process this GO :0070301 and cellular response to hydrogen peroxide and biological process this GO :0070830 and tight junction assembly and biological process this GO :0071277 and cellular response to calcium ion and biological process this GO :0071479 and cellular response to ionizing radiation and biological process this GO :0072686 and mitotic spindle and cellular component this GO :0097149 and centralspindlin complex and cellular component this GO :0097190 and apoptotic signaling pathway and biological process, this GO :0004871 : signal transducer activity, this GO :0004871 : signal transducer activity and also this GO :0005085 : guanyl-nucleotide exchange factor activity and also this GO :0005089 : Rho guanyl-nucleotide exchange factor activity and also this GO :0005096 : GTPase activator activity and also this GO :0005515 : protein binding and also this GO :0017048 : Rho GTPase binding and also this GO :0042803 : protein homodimerization activity, this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0005089 : Rho guanyl-nucleotide exchange factor activity, this GO :0005096 : GTPase activator activity, this GO :0005515 : protein binding, this GO :0017048 : Rho GTPase binding, this GO :0042803 : protein homodimerization activity, 28366 and IDBG-636155 and ENSBTAG00000023814 and 100849477, 504746, epithelial cell transforming sequence 2 oncogene |
| Identity: |
3155 |
| Gene: |
ECT2 |
More about : ECT2 |
| Long gene name: |
epithelial cell transforming 2 |
| Synonyms gene name: |
epithelial cell transforming sequence 2 oncogene |
| Locus: |
3q26, 31 |
| Discovery year: |
1993-10-15 |
| GenBank acession: |
AA206473 |
| Entrez gene record: |
1894 |
| Pubmed identfication: |
8464478 10579713 |
| RefSeq identity: |
NM_018098 |
| Classification: |
Rho guanine nucleotide exchange factors |
| Havana BLAST/BLAT: |
OTTHUMG00000156762 |